Recombinant thermostable enzyme which forms non-reducing sacchar

Details Recombinant thermostable enzyme which forms non-reducing sacchar Recombinant thermostable enzyme which forms non-reducing sacchar

1997-04-11

1999-07-13

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 96, 435 99, 435101, 435200, 435201, 435202, 435205, C12P 1918, C12P 1916, C12P 1914, C12N 926

Patent

active

059225785

ABSTRACT:
Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 69,000-79,000 daltons and a pI of about 5.4-6.4, and forms non-reducing saccharides having a trehalose structure as an end unit from reducing amylaceous saccharides having a degree of glucose polymerization of at least 3. The enzyme has satisfactorily high thermostability, i.e. it is substantially not inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of such non-reducing saccharides on an industrial scale and in a satisfactorily-high yield.

REFERENCES:
patent: 4521252 (1985-06-01), Miyake et al.
patent: 5714368 (1998-02-01), Nakada et al.
Sambrook et al., Molecular Cloning: A Labratory Manual; Cold Spring Harbour Labratory Press; pp. v-xxxii; 1989.
Southern, E.M.; "Detection of Specific Sequences Among DNA Fragments Sepearted by Gel Electrophoresis."; Journal of Molecular Biology; vol. 98; pp. 503-517; 1975.
The Amylase Research Society of Japan, editors; Handbook of Amylases and Related Enzymes: Their Sources, Isolation Methods, Properties and Applications. Permagon Press; pp. xi-81; 1988.
Lama, Licia et al., "Thermostable amylolytic activity from sulfolobus solfataricus." Biotech from Europe, vol. 8, No. 4, pp. 201-203 (1991).
Lama, Licia et al., "Starch conversion with immobilized thermophilic archaebacterium sulfolobus solfataricus." Biotechnology Letters, vol. 12, No. 6, pp. 431-432 (Jun. 1990).

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