Indirect solid surface test for antigens or antibodies

Details Indirect solid surface test for antigens or antibodies Indirect solid surface test for antigens or antibodies

1976-05-10

1978-01-10

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 23259, 424 12, G01N 3300, A61K 4300

Patent

active

040679594

ABSTRACT:
An indirect method for the quantitation of an antigen or antibody in a liquid sample. In the antigen assay, antigen of the same type as the sample antigen is adsorbed onto a solid support surface. Then, specific labelled antibody is immunologically reacted in solution with the sample antigen and the adsorbed antigen. The quantity of reacted labelled antibody on the solid support surface is then determined, as for example, fluorogen label by a fluorometer. The procedure is employed for detection of antibody by reversing the antigen and antibody components in each instance.

REFERENCES:
patent: 3720760 (1973-03-01), Bennich et al.
patent: 3896217 (1975-07-01), Johnson
patent: 3940475 (1976-02-01), Gross
Crosignani et al., Journal of Clinical Endocrinology and Metabolism, vol. 30, No. 2, Feb., 1970, pp. 153-160.
Miles et al., Analytical Biochemistry, vol. 61, No. 1 Sept., 1974, pp. 209-224.
Catt, Acta Endocrinologica Supplementum, No. 142, 1969, pp. 222-246.

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