Method and apparatus for determining the total protein content o

Details Method and apparatus for determining the total protein content o Method and apparatus for determining the total protein content o

1977-01-28

1979-01-23

McGraw, Vincent P.

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

250461B, G01J 330, G01N 2138

Patent

active

041358163

ABSTRACT:
Method for determining the total protein content or individual amino acids in flour, cereal flour and/or leguminous products or other substances by means of fluorescence analysis. The proteins and/or the individual amino acids are treated to attain a fluorescent effect or are self-fluorescent. The proteins and/or the individual amino acids are brought into suspension in their unpretreated form. The suspension is then fluorometrically measured by means of vertical transillumination. An apparatus is provided for practicing the method and comprises a primary radiation source for emitting a primary radiation to excite the fluorescent material, optical means for directing the primary radiation in a vertical direction onto the proteins and/or amino acids in suspension, at least one vessel for holding the suspension, through which the primary radiation can pass in a vertical direction, and receiving means and evaluating means for measuring the fluorescent radiation.

REFERENCES:
patent: 3971951 (1976-07-01), Rikukawa et al.
patent: 3973129 (1976-08-01), Blumberg et al.
patent: 4036946 (1977-07-01), Kleinerman
patent: 4055768 (1977-10-01), Bromberg
"Meas. of Fluorescent . . . Spectrophotometer"; Huke et al.; Josa; vol. 43; May 1953, pp. 400-404.

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