Process for preparing oligopeptide

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525231, 435 698, 935 9, 935 11, 935 14, 935 74, C12P 2100, C12P 2102, C12N 120, C12N 1500

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049353519

ABSTRACT:
An oligopeptide is prepared by use of a vector wherein DNA coding for the intended oligopeptide has been introduced into a plasmid including a leader gene and an alpha-amylase structural gene.

REFERENCES:
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patent: 4565785 (1986-01-01), Gilbert et al.
Kumarev, V. et al, FEBS Letters, vol. 114, No. 2, pp. 273-277, Jun. 1980.
Villa-Komaroff, L. et al, Proc. Natl. Acad. Sci., vol. 75, pp. 3727-3731, 1978.
Cloning and Expression of a Bacillus coagulans Amylase Gene in Escherichia coli, MGG, Mol Gen Genet 1982, 186(4), pp. 507-511.
A Bacillus subtilis Secretion Vector System Derived from the B. subtilis .alpha.-Amylase Promoter and Signal Sequence Region, and Secretion of Escherichia coli .beta.-Lactamase by the Vector System, Journal of Biochemistry, vol. 95, No. 1, 1984, pp. 87-93.
Bacillus subtilis Secretion Vectors for Proteins and Oligopeptides Constructed from B. subtilis .alpha.-Amylase Genes, Mol Biol Microb Differ, Proc Int Spore Conf, 9th 1984 (Pub 1985), pp. 117-123.
Nucleotide Sequence of the Promoter and NH.sub.2 -Terminal Signal Peptide Region of Bacillus subtilis .alpha.-Amylase Gene Cloned.sup.2 in pUB110, Biochemical and Biophysical Research Communications, vol. 112, No. 2, 4/29/83, pp. 678-683.

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