Treatment of paraffin embedded tissue for gene analysis

Details Treatment of paraffin embedded tissue for gene analysis Treatment of paraffin embedded tissue for gene analysis

1995-07-05

1997-09-30

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 254, 536 2541, C07H 106, C07H 108

Patent

active

056726961

ABSTRACT:
Rapid and easy preparation of a sample for a gene analysis or high-purity nucleic acid suitable for gene amplification especially, for example, by the PCR method is made possible by a process for treating a paraffin-embedded tissue sample to be used for a gene analysis, which comprises heating an aqueous suspension containing a surfactant having a protein-denaturational action and a deparaffinized tissue sample obtained from a paraffin-embedded tissue sample at 60.degree. C. or higher, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, mixing the resulting reaction solution with a solution containing an organic compound having a protein-denaturational action other than the aforesaid surfactant, and precipitating nucleic acid from the resulting reaction solution by addition of an alcohol.

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