Cycle labeling and sequencing with thermostable polymerases

Details Cycle labeling and sequencing with thermostable polymerases Cycle labeling and sequencing with thermostable polymerases

1996-09-16

1998-03-03

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

057232989

ABSTRACT:
The present invention provides a novel method for labeling and sequencing nucleic acid molecules, particularly DNA molecules in which an internally labeled, partially extended primer is elongated in a cycled primer extension reaction. An unlabeled DNA primer is contacted with a DNA template in the presence of suboptimal amounts of four dNTPs, one of which is labeled with a detectable marker which may be a fluorescent or visible fluorophor, and infared fluorophor or a radioactive label. This small, labeled primer extension product is then transferred to a new reaction where chain terminated primer extension products for DNA analysis are prepared.

REFERENCES:
patent: 5593830 (1997-01-01), Santamaria et al.
Gyllensten PCR-Technology 1990, Henry A. Edrlich (editor), 1990.
Middendorf et al, Electrophoresis 13:487-494, 1992.
Lee, DNA & Cell Biol. 10(1):67-73, 1991.

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