Isolated native primate GM-CSF protein

Details Isolated native primate GM-CSF protein Isolated native primate GM-CSF protein

1994-11-23

1999-04-20

Mertz, Prema

Drug, bio-affecting and body treating compositions

Lymphokine

530351, 530412, 514 2, 514 8, 514 12, 930145, C07K 14535, C07K 126

Patent

active

058956468

ABSTRACT:
A method for purifying CSF protein is described. The method comprises: precipitating the protein with ammonium sulfate at 80% saturation to form a pellet containing the CSF protein; resuspending the pellet in a buffered solution at a pH in the range of about 6 to about 8; applying the buffered solution containing CSF to a chromatographic column, eluting with the buffered solution containing sodium chloride and collecting the fractions having CSF activity; pooling the active fractions, applying them to a C4 reverse phase column and eluting with a 0 to 90% acetonitrile gradient to collect the active fractions. The purified CSF protein has a specific activity of at least about 1.times.10.sup.7 units per mg of protein and preferably at least about 4.times.10.sup.7 units per mg of protein when assayed using the human bone marrow assay.

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