Use of the protease domain of human plasminogen activator for th

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing

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424 9464, 435216, 435217, 435219, A61K 3843, A61K 3848, C12N 948, C12N 970

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active

057231222

ABSTRACT:
The invention relates to the use of thromboembolic proteins. These contain, as their only structural portion effecting thrombolytic activity, the protease domain of human tissue type plasminogen activator. These derivatives show reduced side effects, such as a reduction in bleeding while. demonstrating remarkable in vivo efficacy. The effect is surprising, given their in vitro properties.

REFERENCES:
patent: 5223256 (1993-06-01), Stern et al.
patent: 5242688 (1993-09-01), Burck et al.
Burck et al., "Characterization of a Modified Human Tissue-Plasminogen Activator Comprising a Kringe-2 and a Protease Domain", J. Biol. Chem. 265 (9): 5170-5177 (1990).
Robinson et al., "Redesigning t-PA for improved thrombolytic-therapy", Trends Biotech. 9 (3): 86-90 (1991).
Gething et la., "Variants of Human Tissue Type Plasminogen-Activator That Lack Specific Structural Domains of the Heavy Chain", EMBO J 7(9): 2731-2740 (1988).
Stockinger et al., 1992, Thrombosis Res., vol. 67, pp. 589-599.
Konnert et al., 1992, Protein Eng., vol. 5, No. 1, pp. 93-100.

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