Monoclonal antibodies and method of identifying species using th

Details Monoclonal antibodies and method of identifying species using th Monoclonal antibodies and method of identifying species using th

1985-07-16

1988-04-05

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 13, 435 68, 43524027, 435810, 436547, 436548, 436518, 436528, 436808, 436810, 935103, 935104, 935105, 935110, G01N 3353, G01N 33577, C12N 1500

Patent

active

047358980

ABSTRACT:
The new method an products recognize human serum albumin, differentiating blood and tissue from human sources from blood and tissue of other animals. The method and products are particularly useful in forensic investigations. First, mice are injected with purified human serum albumin, spleen cells from the mice are fused with cells from a murine myeloma. Resulting hyberdomas are screened on human serum albumin coated plates. Subcloning, stabilizing by culture and continued assays for anti-human albumin activity produces products which recognize and differentiate blood or tissue samples from samples of other species.

REFERENCES:
Fletcher et al, Chemical Abstracts, 100(1984) 115935, Abstracting J. Forensic Sci. 1984, 29(1) 67-74.
Watanabe et al, Chemical Abstracts, 103(1985) 99821y Abstracting Kanagawa Keisatsu Kenkyusho Hokoku, Hokagaku Hen, 1984, 37(3) 169-75 (Japan).
Lapresle et al, Molecular Immunology, vol. 20(5), pp. 549-555 (1983).
Doyen et al, Molecular Immunology, vol 22(1), pp. 1-10 (1985).
Hosoi et al, Clinical Immunological Immunopathology, vol. 32(3), Abstract only (1984).

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