Monolayer device using filter techniques

Details Monolayer device using filter techniques Monolayer device using filter techniques

1981-05-14

1983-07-26

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

356 38, 422 66, 435287, 435291, 435808, C12M 136, C12M 100, C12M 134, G01N 100

Patent

active

043954936

ABSTRACT:
Disclosed is a monolayering device comprising a cell sensor arrangement for receiving a liquid suspension of cells and for controlling the number of cells passing through to an application vessel, a filter tape having the application vessel positioned thereabove at a first station, a vacuum source for sucking the liquid of the liquid suspension from the application vessel through the tape, a tape moving arrangement for moving the portion of the tape having the cells deposited thereon from the first station to a second station whereat a slide is positioned, and a sponge having a fixative therein mounted on a block for biasing the tape against the slide so that the cells will adhere to the slide.

REFERENCES:
patent: 3653773 (1972-04-01), Childs
patent: 3654091 (1972-04-01), Binnings et al.
patent: 3690832 (1972-09-01), Plakas
patent: 3902971 (1975-09-01), Fletcher et al.
patent: 3940250 (1976-02-01), Plakas et al.
patent: 4084902 (1978-04-01), Green
patent: 4321122 (1982-03-01), Whitcomb et al.
Garcia, G. L., and Tolles, W. E., "Ultrasonic Disaggregation of Cell Clusters", J. Histochem. Cytochem., 25:508, 1977.
Rosenthal, D. L., Stern, E., McLatchie, C. A., Lagasse, L. D., Wall, R., and Castleman, K. R., "A Simple Method of Producing a Monolayer of Cervical Cells for Digital Image Processing", Anal. Quant. Cytol., 1:84, 1979.

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