Assay for verticillium dahliae

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, C12Q 168, C12P 1934

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055276717

ABSTRACT:
A DNA sequence is described which is diagnostic of Verticillium dahliae, a problematic plant pathogen. The sequence is found only in V. dahliae and not in closely related Verticillium species. The identification of this diagnostic DNA sequence permits a rapid and sensitive PCR assay to be used to test soil samples for the presence of this pathogen. Competitive PCR may be used to quantify the amount of pathogen present.

REFERENCES:
Atlas et al. (1990) pp. 399-406 in "PCR Protocols" Ed. Innis et al. Academic Press, Inc.
Nazar et al. (1991) Physiol. and Molec. Plant Pathology 39: 1-11.
Young et al. (1993) Appl. and Environ. Microbiol. 59(6): 1972-1974.
Gilliland et al. (1990) pp. 60-69 in "PCR Protocols" Ed. Innis et al. Academic Press, Inc.

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