Islet isolation process

Details Islet isolation process Islet isolation process

1989-06-09

1994-06-21

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435267, 435 1, 4352401, C12S 324

Patent

active

053227905

ABSTRACT:
A method of producing intact islets of Langerhans in an insulin producing condition uses a mixture of Hank's solution and 10% by volume fetal calf serum to ductally distend the human pancreas. The exocrine tissue of the pancreas is digested at about 37.degree. C. by an enzyme preparation of collagenase, trypsin and proteolytic enzyme present in the mixture at a level of about 0.2% by weight. The digestion is monitored at regular intervals during the process. The digested pancreas is comminuted, filtered and intact islets of Langerhans are recovered. The recovered islets retain their insulin producing properties.

REFERENCES:
patent: 4868121 (1989-09-01), Scharp et al.
Lacy, P. E., et al., Diabetes, 31(8):109-111 (1982).
Scharp, D. W., et al., Diabetes, Supplement 1, 29(2):19-30 (1980).
Downing, R., et al., Brief Comm., 29(1):79-83 (1980).
Horaguchi, A., et al., Diabetes, 30: 455-458 (1981).
Shibata, A., et al., Diabetes, 25(8):667-762 (1976).
Horaguchi, A., et al., J. Surg. Res., 35:277-282 (1983).
Lindall, A., et al., Endocrinology, 85:218-223 (1969).
Jakoby, et al. (Eds.) Methods in Enzymology, vol. LVIII, pp. 119-131, Academic Press, N.Y., 1979.
Sutherland, et al., J. Surg. Res., 16:102-111 (1974).

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