DNA extraction buffer and method of use thereof

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435410, 435803, 536 254, C12Q 168, C07H 2100, C12P 1934, C12N 502

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active

060716983

ABSTRACT:
Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques. Also described is a novel extraction buffer solution for use in isolating DNA from an organism.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4828890 (1989-05-01), Tiedeman
patent: 5324632 (1994-06-01), Weisburg et al.
patent: 5338745 (1994-08-01), Fukazawa
patent: 5447848 (1995-09-01), Barns et al.
patent: 5558996 (1996-09-01), Bartlett
patent: 5585238 (1996-12-01), Ligon et al.
patent: 5612208 (1997-03-01), Nakanishi
patent: 5834250 (1998-11-01), Wells
patent: 5871975 (1999-02-01), Kacian
Cheung,W. et al. PCR Methods and Applications 3(1):69-70, 1993.
Chunwongse, J. et al. Theor. Appl. Genet. 86:694-698, 1993.
Kreader, C. Appl. Envir. Midrobiol. 62(3):1102-1106, Mar. 1996.
Sigma Catalog, p. 62-65, 1993.
Steiner, J.J. et al. Nucleic Acids Research 23(13):2569-2570, Jul. 1995.
Basha, S.Y. et al. Current Science 68(6):587-588, Apr. 1995.
Bentolila, et al., "Identification of an RFLP marker tightly linked to the Ht1 gene in maize", Theo. Appl. Genet, 82:393-398 (1991).
Johanson et al. "Use of PCR for detection of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka leaf spots in banana and plantain", Mycol. Res., 97:670-674 (1993).
Hung et al., "A specificity enhancer for polymerase chain reaction", Nucleic Acids Research, 18(16): 4953 (1990).
Klimyuk et al., "Alkali treatment for rapid preparation of plant material for reliable PCR analysis", The Plant Journal, 3(3): 493-494 (1993).
Nazar, R.N., et al, "Potential use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of verticillium wilt pathogens", Physiol. and Molec. Plant Pathol., 39:1-11 (1991).
Oliver et al., "Amplification and sequencing of ribosomal DNA ITS regions for specific detection of Helminthosporium solani", Phytopathology 86(11) pp. S1 and S12 (Supplement) (1996).
Poupard et al., "Molecular characterization of Pseudocercosporella herpotrichoides isolates by amplification of ribosomal DNA internal transcribed spaces", Plant Pathology, 42: 873-881 (1993).
Schesser et al., "Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants", Applied and Environ. Microbiol., 57(2):553-556 (1991).
Stratagene Catalog, 1988, p. 39.
Tisserat et al., "Selective Amplification of rDNA Internal Transcribed Spacer Regions to Detect Ophiosphaerella korrae and O. herpotricha", Phytopathology, 84(5): 478-482 (1994).
Wang, et al., "A simple method of preparing plant samples for PCR", Nucleic Acids Research, 21(17): 4153-4154 (1993).
White, T.J., et al., "Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics", In: PCR Protocols; Academic Press Inc., pp. 315-322 (1990).
GenBank Accession No. L08734, computer printout, Feb. 5, 1993.
Xue et al., "Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences", Physiological and Molecular Plant Pathology, 179-188 (1992).

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