Method and test kit for the rapid detection of biotoxic contamin

Details Method and test kit for the rapid detection of biotoxic contamin Method and test kit for the rapid detection of biotoxic contamin

1994-03-23

1997-03-25

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 26, 435 25, 435 18, 435 15, 435 24, 435 14, 435 4, 435242, 435243, 4352525, 435834, 435836, 435839, 435837, 435975, 435838, C12Q 102, C12Q 132, C12M 300, C12M 120

Patent

active

056143753

ABSTRACT:
The invention provides a method for the rapid detection of biotoxic contaants in a test material comprising combining a sample of the test material with activated spores essentially devoid of detectable enzymatic activity, which activity becomes manifest and increases measurably following germination of the spores and with at least one germinant capable of triggering germination of the spores and a substrate which is catalytically convertible to a product by the enzymatic activity, incubating the mixture for a period of less than one hour to accelarate germination of the spores, increase of the enzyme activity, and a catalytic conversion of the substrate, and detecting the formation of the product of the catalytic conversion of the substrate and the enzyme, the level of the product being maximal in the absence of any biotoxic contaminants, and decreasing in direct proportion to the toxicity of contaminants in the test sample.

REFERENCES:
patent: 4381343 (1983-04-01), Citri
patent: 4603108 (1986-07-01), Bascomb
patent: 4774173 (1988-09-01), Reinhartz
patent: 4808517 (1989-02-01), Blondin et al.
patent: 5019411 (1991-05-01), Johnson et al.
patent: 5149656 (1992-09-01), Bitton
patent: 5260213 (1993-11-01), Harman et al.
J. Gen. Microb., Venkatasubramanian et al, vol. 135, pp. 2723-2733, 1989.
Kniest et al (Abstract) Biotechnology Techniques, vol. 4 (4) p. 269, (1990) .

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