Drug – bio-affecting and body treating compositions – Lymphokine – Interleukin
Patent
1995-04-25
1997-03-25
Fitzgerald, David L.
Drug, bio-affecting and body treating compositions
Lymphokine
Interleukin
530351, 530415, 435 6952, A61K 3820, C12N 1526
Patent
active
056141858
ABSTRACT:
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the-cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
REFERENCES:
patent: 3897309 (1975-07-01), Grabner
patent: 4275056 (1981-06-01), Takaku et al.
patent: 4289690 (1984-12-01), Pestka et al.
patent: 4315852 (1982-02-01), Leibowitz et al.
patent: 4390623 (1983-06-01), Fabricius et al.
patent: 4401756 (1983-08-01), Gillis
patent: 4448879 (1984-05-01), Fabricius et al.
patent: 4450103 (1984-05-01), Konrad et al.
patent: 4462940 (1984-07-01), Hanisch et al.
patent: 4464295 (1984-08-01), Bhaduri et al.
patent: 4464355 (1984-08-01), Fabricius et al.
patent: 4476049 (1984-10-01), Kung
patent: 4490289 (1984-12-01), Stern et al.
patent: 4511502 (1984-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518584 (1985-05-01), Mark et al.
patent: 4530787 (1985-07-01), Shaked et al.
patent: 4564593 (1986-01-01), Tsukamoto et al.
patent: 4569790 (1986-02-01), Koths et al.
patent: 4572798 (1986-02-01), Koths et al.
patent: 4582799 (1986-04-01), Jarvis, Jr.
patent: 4675383 (1989-06-01), Bohlen et al.
patent: 4738927 (1988-04-01), Taniguchi et al.
patent: 4778879 (1988-10-01), Merkels et al.
patent: 4782139 (1988-11-01), DiMarchi et al.
patent: 4787658 (1988-12-01), Yoshimoto et al.
patent: 4925919 (1990-05-01), Mertelsmann et al.
patent: 4992271 (1991-02-01), Fernandes et al.
patent: 5015730 (1991-05-01), Friesen et al.
patent: 5156968 (1992-10-01), Liu
patent: 5419899 (1995-05-01), Koths et al.
Berman et al., Trends Biochem., 3(2), 51-53 (1985).
Coughlin et al., Federation Proceedings, 42(7), 2022 (1983).
Devos et al., Nucleic Acid Res., 11, 4307 (1983).
Derynck et al., Nature, 287, 193-197 (1980).
Doyle et al., J. Biol. Resp. Mod., 4, 96-109 (1985).
Gillis et al., J. Immunol., 124 (4), 1954-1962 (1980).
Hearn et al., High Performance Liquid Chromatography of Proteins and Peptides Academic Press (1982).
Henderson et al., J. Immunol., 131(2), 810-815 (1983).
Henriksen et al., Cell. Immunol., 73, 106-114 (1982).
Hisashi et al., Chemical Abstract, 74 (10,) Abstract 45571f, p. 226 (1971).
Hochauser et al., High Technology, 55-60 (1983) (partial copy).
Hostettmann et al., Preparative Chromatography Techniques, Springer-Verlag, New York (1986).
Ihle et al., J. Immunol., 129, 2431 (1982).
Lahm et al., J. Chromatography, 326, 357-361 (1985).
Lewis et al., Anal. Biochem., 104, 153-159 (1980).
Lewis et al., J. Liquid Chromatography, 5(7), 1367-1374 (1982).
Lymphokine Res., 1(2), Editorial: FASEB (1982).
Mier et al., J. Immunol., 128(3), 1122-1127 (1982).
Milestone et al., Biochem. Biophys. Res. Comm., 115(2), 762-768 (1983).
Mochizuki et al., J. Immunol. Meth., 39, 185-201 (1980).
Pearson et al., Anal. Biochem., 124, 217-230 (1982).
"Points to consider in the production and Testing of New Drugs and Biologicals Produced by Recombinant DNA Technology," Office of Biologics Research & Review (1983).
Regnier, Science, 222(4621), 245-249 (1983).
Riendeau et al., J.B.C., 258(20), 12114-12117 (1983).
Robb et al., Mol. Immunol., 18(12), 1087-1099 (1981).
Robb et al., Proc. Nat'l. Acad. Sci. (USA) 80: 5000-5004 (1982).
Robb, Immunol. Today, 5(7), 203 (1984).
Robson, et al., ed., Introduction to Proteins and Protein Engineering, pp. 28-32 (1986).
Rosenberg et al., Science, 223, 1412-1415 (1984).
Rosenberg et al., New Eng. J. Med., 313, 1485-1492 (1985).
Sofer, Biotechnology, 1035-1038 (Dec. 1984).
Sofer et al., BioTechniques, 198-203 (Dec. 1983).
Stern et al., Proc. Nat'l. Acad. Sci. (USA), 81, 871-875 (1984).
Taniguchi et al, Nature, 302, 305-309 (1983).
Wang et al., Science, 224, 1431-1433 (1984).
Watson et al., J. Exp. Med., 150, 849-861 (1979).
Wells et al., Gene, 34, 315-323 (1985).
Welte et al., J. Exp. Med., 156, 454-464 (1982).
Sharma, Journal of Biotechnology and Applied Biochemistry (1986) 8:5-22.
Sofer, Bio/Technology, (1984) pp. 1035-1038.
McIntire et al., Lipopolysaccharide Inactivation by Deoxychlate pp. 4063-4066.
Brochure from Pierce Chemical Co..
Hanisch Wolf
Koths Kirston
Kunitani Michael
Thomson James
Wilson Kenneth
Blackburn Robert P.
Chiron Corporation
Fitzgerald David L.
McGarrigle, Jr. Philip L.
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