Method and reagent for the determination of iron

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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436 43, 436 73, 436 84, 436166, 436174, 436175, 422 56, 422 61, G01N 3352, G01N 3390

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active

057632812

ABSTRACT:
In order to determine iron in serum by releasing the bound iron through addition of a protein denaturing agent, reduction of the released iron to Fe.sup.2+, addition of a color reagent solution and photometric measurement of the color complex that forms, a mixture of a denaturing agent containing urea or a urea derivative and a fatty alcohol polyglycol ether are added to the sample solution.

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S. Tajima et al. Arch. Biochem. Biophys. 1979, 198, 137-144.
H. Hoffmann et al. J. Colloid. Interfac. Sci. 1981, 80, 237-254.
R. Jha et al J. Chem. Soc., Faraday Trans. 1993, 89, 3465-3469.

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