Immobilized phosphorylase

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435193, 435194, 435105, 435181, C12N 1108, C12N 910

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active

055433103

ABSTRACT:
A process for preparing glucose-1-phosphate from an .alpha.-glucan and orthophosphate using a phosphorylase immobilized onto an anion-exchange resin is disclosed. The anion-exchange resin used is a synthetic polymer resin into which an anion exchange group; a primary to quarternary ammonium group, a phosphonium group, or a sulfonium group, is introduced. This type of immobilized phosphorylase ensures (i) an effective utilization of phosphorylase, (ii) a higher reactivity of a higher absorbance of the phosphorylase to the resin, (iii) an excellent phosphorylase activity retention performance, and (iv) a higher physical strength of the resin.

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