Method for immunoselection of cells using avidin and biotin

Details Method for immunoselection of cells using avidin and biotin Method for immunoselection of cells using avidin and biotin

1991-10-03

1993-11-16

Lacey, David L.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

424 2, 4351722, 435177, 4352401, 436501, 436538, 5303884, 53038873, C12N 1300, C12N 500, A61K 3514, G01N 33530

Patent

active

052623343

ABSTRACT:
The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.

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