Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1993-03-12
1996-06-25
Housel, James C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530412, 530415, 530427, 530825, 4242341, C07K 116, C07K 118, C07K 122
Patent
active
055301037
ABSTRACT:
A method of purifying B. burgdorferi proteins comprising the steps of (a) disrupting a B. burgdorferi cell and fractionating the disrupted cell into membrane and cytoplasmic components; (b) resuspending the membrane component in a non-denaturing detergent thereby producing a solubilized protein and an insolubilized material and then separating said solubilized protein from said insolubilized material; (c) subjecting the solubilized protein to ion-exchange chromatography so as to produce protein fractions; and (d) assaying the protein fractions to identify those fractions which contain proteins of interest wherein the purified proteins of interest are in a biologically active form suitable for use in vaccines, is disclosed.
REFERENCES:
patent: 4721617 (1988-01-01), Johnson
patent: 4888276 (1989-12-01), Shelburne
patent: 5192540 (1993-03-01), Kuo et al.
Dunn et al Protein Expression & Purification 1: 159-168 1990.
Brandt et al, Infect & Immun. 58(4): 983-991, 1990.
Praec Mursic et al. Infection, 18: 332-341 (1990).
Barbour Clin. Microbiol. Revs., 1(4): 399-414 (1988).
Barbour Rev. Inf. Dis., 11(6): S1470-1474 (1989).
Bergstrom et al. Mol. Microbiol., 3(4): 479-486 (1989).
Wilske et al. N.Y. Acad. Sci., 539: 126-143 (1988).
Gassman et al. Nuc. Acids Res., 17(9): 3590 (1989).
Simpson et al. J. Clin. Microbiol., 28(6): 1329-1337 (1990).
Fuchs et al. Fourth Intern. Conference on Lyme Borreliosis (1990).
Wilske et al. Zbl. Bakt. Hyg., 263: 92-102 (1986).
Hansen et al. J. Clin. Microbiol., 26(2): 338-346 (1988).
Coleman et al. J. Infect. Dis., 155(4): 756-765 (1987).
Wilske et al. Fourth Intern. Conf. on Lyme Borreliosis (1990).
Fikrig et al. Science, 250: 553-556 (1990).
Regenmortel Immunology Today, 10(8): 226-272 (1989).
Simpson et al, National Technical Info. Service Accession #PB90-206376, U.S. patent application Ser. No. 07/487,716, filed Mar. 5, 1990 Published by Dept. of Health & Human Services.
Schaible et al. PNAS, U.S.A., 87: 3768-3772 (1990).
Steere The New England Journal of Medicine, 321(9): 586-596 (1989).
Luft et al. Infection and Immunity, 57(11): 3637-3645 (1989).
Wilske J. of Infectious Diseases, 153(2): 304-314 (1986).
German Patent Application, Max Plankt Institute, published Mar. 19, 1991.
Berzofsky et al. "Protein Antigenic Structures Recognized by T Cells: Potential Applications to Vaccine Design", Immunological Reviews, 98: 9-52 (1987).
Simon et al. "A mouse model for Borrelia burgdorferi infection: approach to a vaccine against Lyme disease", Immunology Today, 12(1): 12-16 (1991).
Simpson et al. National Technical Info. Service Accession #PB90441144, U.S. patent application Ser. No. 07/427,735, filed Oct. 26, 1989 Published by Natl Inst. Heath.
Doward et al, National Technical Info Service Accession #PB90-213018, U.S. patent application Ser. No. 07/485,551 filed Feb. 27, 1990. Published by Natl. Inst. Health.
Schwan et al, National Technical Info. Service Accession #PB90-134841, U.S. Patent Application 07/361,850 filed Jun. 5, 1989, published by Dept of Health & Human Services.
Data For Biochemical Research 3rd Edition, Dawson et al eds. Clarendon Press, Oxford, 1987 p. 287.
Cull et al, "Preparation of Extracts from Prokaryotes" in Methods in Enzymology 182: 147-153, 1990.
England et al, "Precipitation Techniques" in Methods in Enzymology 182: 285-300, 1990.
Gondolf et al J. of Chromatography 521:325-334, 1990.
Gabay et al, J. Bacteriology 162(1): 85-91, 1985.
Benz R. Crit Rev. Biochem 19:145-190, 1985.
Helenius et al Biochimica et Biophysica Acta 415: 24-79, 1975.
Wilske et al, Res Microbiol, 143: 583-596, 1992.
Dorner Friedrich
Livey Ian
Housel James C.
Immuno Aktiengesellschaft
Krsek-Staples Julie
LandOfFree
Method for the purification of PC protein from Borrelia burgdorf does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the purification of PC protein from Borrelia burgdorf, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the purification of PC protein from Borrelia burgdorf will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2189841