Lactococcus promoters and signal sequences for heterologous gene

Details Lactococcus promoters and signal sequences for heterologous gene Lactococcus promoters and signal sequences for heterologous gene

1993-02-10

1996-06-25

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 6, 435 712, 4351723, 4352523, 43525231, 43525233, 4353201, 536 231, 536 241, C12N 1574, C12N 121

Patent

active

055299083

ABSTRACT:
DNA sequences, derived from Lactococcus lactis subsp. lactis, are useful as promoters and promoter/secretion promoting signals for heterologous or homologous expression in Gram-positive bacteria. In another aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and to host cells transformed with such vectors. Yet additional aspects of the present invention are related to methods for producing desired heterologous or homologous peptides or proteins employing the sequences, vectors, or transformed hosts of the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion may be achieved in E. coli and in Gram-positive bacteria such as B. subtilis, Lactococcus and Lactobacillus.

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