Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1993-11-18
1996-06-25
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 25, 435 26, 435189, 435190, 435810, 435975, C12Q 142, G01N 3353
Patent
active
055299067
ABSTRACT:
A system or kit for analysis of phosphatase comprising a reagent I containing NADP or NADPH as a main component, and a reagent II containing components for color-development signal amplification utilizing NAD-NADH interconversion, wherein at least one of the hydrogen donors participating in the NAD-NADH interconversion is included in reagent I, but not in reagent II, which eliminates the necessity that the reagent II is further separated into two parts. This enables the preparation of reagent II as a mixture which can be readily used and which can be preserved for a long period of time without "reagent blank" occurring. Additionally, the present kit eliminates the necessity of mixing reagents I and II immediately before actual use and thus permits periodical analysis of samples with a reagent having the same components for long time periods with reliable and uniform sensitivity.
REFERENCES:
patent: 4490465 (1984-12-01), Limbach et al.
patent: 4769321 (1988-09-01), Self
patent: 5272054 (1993-12-01), Switchenko et al.
Khalmuratov et al, Biological Abstracts, vol. 73, No. 2, p. 914, Ref. #8882, 1981 (Ukr. Biokhim. Zh. 52(6):742-745, 1980).
Lovgren et al, Analytica Chemica Acta, vol. 288, pp. 227-235, 1994.
Shimizu Akiko
Shinzaki Akihiro
Suzuki Tadao
Watanabe Miwa
Mitsubishi Cable Industries Ltd.
Mohamed Abdel A.
Wityshyn Michael G.
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