Method for the determination of .alpha.-amylase

Details Method for the determination of .alpha.-amylase Method for the determination of .alpha.-amylase

1980-09-08

1982-06-29

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

536106, C12Q 140

Patent

active

043373100

ABSTRACT:
Method for determining .alpha.-amylase by incubating with a starch substrate, wherein the substrate is based upon particulate starch derivatized with detectable groups for the determination of .alpha.-amylase, the substrate consisting of superficially cross-linked starch or amylose grains having a regulated, reduced swellability, said grains having a diameter of from 0.01 to 0.20 mm; these grains are prepared by suspending non-swollen grains in a solution with only enough cross-linking agent to achieve superficial cross-linking and then derivatizing the grains with the detectable groups for the determination of .alpha.-amylase.

REFERENCES:
patent: 2500950 (1950-03-01), Konigsberg
patent: 3525672 (1970-08-01), Wurzburg et al.
patent: 3597322 (1971-08-01), Babson
patent: 3759794 (1973-09-01), Sax et al.
patent: 4025392 (1977-05-01), Dougherty
Ewen, "Synthesis of Cibachron Blue F3GA-Amylose, Clinica Chimica Acta, Aug. 30, 1973, pp. 233-248.

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