Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-02-14
1997-06-17
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, C12Q 168, C12P 1934
Patent
active
056396082
ABSTRACT:
This invention relates to methods for determining the nucleotide base sequence of a deoxyribose nucleic acid molecule comprising the steps of incubating the nucleic acid molecule with an oligonucleotide primer of 5 to 8 bases in length, a plurality of deoxynucleoside triphosphates, at least one chain terminating agent, and a T7-type DNA polymerase having less than 500 units of exonuclease activity under conditions in which the primer is extended until the chain terminating agent is incorporated, and separating the products of the incubating step according to size, whereby at least a part of the nucleotide base sequence of the nucleic acid molecule can be determined.
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Bart
Richardson Charles C.
Tabor Stanley
Arthur Lisa B.
President & Fellows of Harvard College
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