Method for quantitative determination of renin activity in blood

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Separation of immune complex from unbound antigen or antibody

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435 4, 435 7, 435 18, 435 19, 435269, 435272, 436542, 436811, 436822, 436824, 436825, 436826, G01N 3354, G01N 3356, C12Q 134, C12Q 144

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RE0315176

ABSTRACT:
A method for the quantitative determination of renin activity in blood utilizing the measurement of Angiotensin I by treating the blood with ethylenediaminetetraacetic acid (EDTA) and the plasma with phenylmethyl sulfonylfluoride (PMSF) at a preferred pH to ascertain the full range of concentration where PMSF is effective for accurate and quick determination.
Also, a method for the quantitative determination of renin activity in blood utilizing the measurement of Angiotensin I by incubating the samples after antibody addition at room temperature (23.degree. to 30.degree. C.) for 1 to 2 hours and separating the free from the antibody bound species with polyethylene glycol after having treated the blood with ethylenediaminetetraacetic acid (EDTA) and the plasma with phenylmethyl sulfonylfluoride (PMSF) at a preferred pH. PMSF offering enough protection against Angiotensin I destruction at room temperature that in combination with polyethylene glycol at a preferred pH the time required for the radioimmunoassay determination is 1 to 2 hours at room temperature instead of 24.+-.2 hours at 4.degree. C. which takes with charcoal in the presence of other inhibitors and making the system less succeptible to time dependent errors.

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