Assay for motile facultative anaerobic pathogens

Details Assay for motile facultative anaerobic pathogens Assay for motile facultative anaerobic pathogens

1990-10-09

1993-02-16

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 29, 435 34, 435244, 4352521, 4352536, 435296, 435801, C12Q 126, C12N 120

Patent

active

051870703

ABSTRACT:
An improved assay for the determination for motile facultative anaerobic pathogens is described which involves the use of a growth-enhancing substance such as oxyrase enzyme in order to increase the growth rate of a target pathogen in a growth medium, to thereby shorten the time required to complete the assay. The assay of the invention is particularly suited for the determination of L. monocytogenes in food products such as meat and dairy goods, and provides and effective screening assay which can be completed in about 24-36 hours. Preferably, a product sample is first incubated in Fraser Broth containing oxyrase enzyme; if a darkened color results, a small portion of the liquid is then placed within a U-shaped culture tube provided with modified Oxford agar; the motility characteristics of the target pathogen are observed in the U-tube, which is indicative of the presence of L. monocytogenes.

REFERENCES:
patent: 4996073 (1991-02-01), Copeland et al.
Edberg et al., Journal of Clinical Microbiology, vol. 21, No. 3, pp. 363-365, Mar. 1985.
Cassiday et al., Applied and Environmental Microbiology, vol. 56, No. 7, pp. 2274-2275, Jul., 1990.

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