Thermostable glycerokinases and process for its production

Details Thermostable glycerokinases and process for its production Thermostable glycerokinases and process for its production

1981-07-16

1983-05-24

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435172, 435253, 435832, C12N 912, C12N 1500, C12N 120, C12R 107

Patent

active

043851207

ABSTRACT:
A thermostable glycerokinase enzyme useful in the detection and estimation of glycerol derivatives has a half-life in excess of 1 hour at 55.degree. C. at a protein concentration of less than 2 mg/ml and a pH of 7.8 .+-.0.5 in the absence of any substrate. The enzyme is produced by culturing at least one micro-organism which is capable of growth at a temperature of at least 50.degree. C. in a culture medium in which it will produce said enzyme, disrupting the resulting cells of the micro-organism to release the enzyme and separating the enzyme from the cell debris. The medium normally contains at least 0.1% glycerol but certain strains of micro-organism have been found to be capable of producing thermostable glycerokinase enzyme even in the absence of glycerol. The micro-organism is preferably a Bacillus organism, especially of the stearothermophilus species.

REFERENCES:
Chemical Abstracts, vol. 83, (1975), 129984s.
Lindgren et al., Journal of Bacteriology, Sep. 1976, vol. 127, No. 3, pp. 1047-1057.
Barman, Enzyme Handbook, vol. 1, (1969), pp. 401-402.
Atkinson et al., Journal Appl. Bacteriol., vol. 38, No. 3, pp. 301-304, (1975).

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