Species-specific DNA probes for vibrio cholerae and methods

Details Species-specific DNA probes for vibrio cholerae and methods Species-specific DNA probes for vibrio cholerae and methods

1995-06-07

1997-03-04

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2432, 536 2433, C12Q 168, C07H 2104

Patent

active

056078358

ABSTRACT:
The present invention relates to species-specific DNA probes specific for Vibrio vulnificus and Vibrio cholerae. The DNA probes of the present invention specifically detects Vibrio vulnificus or Vibrio cholerae in a mixed bacterial sample based on unique ribosomal RNA nucleotide sequences. When the DNA probes of the present invention are tagged with a labeled molecule such as a fluorescent label, it affords direct and immediate visualization of individual bacterial cells, and a rapid method of detection of bacterial infection in humans and shellfish without culturing.

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