Antibodies specific for hLH .beta. core fragment

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...

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435326, 435346, 5303871, 53038824, 5303892, C07K 1626, C12N 512

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active

059768762

ABSTRACT:
This invention provides an antibody which specifically binds to hLH.beta.cf without cross-reacting with hLH, hLH.beta. or hCG.beta.cf. In an embodiment, the monoclonal antibody is designated B505. In a further embodiment, the hybridoma cell line producing the monoclonal antibody B 505 is designated ATCC Accession No.12000. This invention provides different uses of the antibodies. Finally, this invention provides a method for determining the amount of hLH.beta.cf or hLH.beta.cf-related molecule in a sample.

REFERENCES:
patent: 4565687 (1986-01-01), Khazaeli et al.
patent: 4804626 (1989-02-01), Bellet et al.
patent: 4851356 (1989-07-01), Canfield et al.
patent: 5356817 (1994-10-01), Cole
patent: 5445968 (1995-08-01), Blithe et al.
patent: 5674983 (1997-10-01), Blithe et al.
Birken, S. et al. Endocrin. 133: 983-989, Sep. 1993.
Iles, R. K. et al. J. Endocrin. 133:459-466, Sep. 1992.
Harlow, E. et al. Antibodies, A Laboratory Manual, CSHL, Cold Spring Harbor, N. Y., pp. 72-77, 92-97, 128-135, and 141-157, 1988.
Weir, D.M. et al. vol. 1 Immunochemistry, Blackwell Scientific Publications, Boston, MA., pp. 8.14-8.15, 1986.
Armstrong, et al. 1984. Use of a highly sensitive and specific immunoradiometric assay for detection of human chorionic gonadotropin in urine of normal, nonpregnant and pregnant individuals. J. Clin. Endocrinol. Met. 59:867-874 (Exhibit A).
Barbe, F. et al. 1995. undetectable luteinizing hormone levels using a monoclonal immunometric assay. J. Endocrinol. Invest. 18(1):806-808 (Exhibit B).
Birken, S., et al. 1993. Structure and significance of human luteinizing hormone-beta core fragment purified from human pituitary extracts. Endocrinology. 133(3):985-989 (Exhibit C).
Blithe, et al. 1988. Purification of .beta.-core fragment from pregnancy urine and demonstration that its carbohydrate moieties differ from those of native human chorionic gonadotropin-.beta.Endocrinology 122: 173-180 (Exhibit D).
Cole, L.H., et al. 1988. Urinary human chorionic gonadotropin free .beta.-subunit and .beta.-core fragment: a new marker of gynecological cancers. Cancer Research 48: 1356-1360 (Exhibit E).
Costagliola, S. et al. 1994. Glycoprotein hormone isomorphism and assay discrepancy: the paradigm of luteinizing hormone (LH). [Review]. J. Endocrinol. Invest. 17(4):291-9(Exhibit F).
Iles, R.K. et al. 1992. Immunoreactive beta-core-like material in normal postmenopausal urine: human chorionic gonadotropin or LH origin? Evidence for the existence of LH core J. Endocrinol. 133 (3): 459-466 (Exhibit G).
Kardana, et al. 1988. Urinary gonadotropin peptide-isolation and purification and its immunohistochemical distribution in normal and neoplastic tissues. British Journal of Cancer 58: 281-286 (Exhibit H).
Krichevsky, A. et al. 1988. Preparation and Characterization of antibodies to the urinary fragment of the human chorionic gonadotropin beta-subunit. Endocrinology 123(1): 584-593 (Exhibit I).
Martin-Du-Pan, R.C. et al. 1994. Clinical significance of invisible or partially visible luteinizing hormone. Hum. Reprod. 9(11):1987-1990 (Exhibit J).
Moyle, W.R. et al. 1982. Use of monoclonal antibodies to subunits of human chorionic gonadotropin to examine the orientation of the hormone in its complex with receptor. PNAS 79: 2245-2249 (Exhibit K).
Neven, P. et al. 1993. Substantial urinary concentrations of material resembling beta-core fragment of chorionic gonadotropin beta-subunit in mid-menstrual cycle. Clin. Chem. 39 (9):1857-1860 (Exhibit L).
O'Connor, et al. 1988. Development of highly sensitive immunoassays to measure human chorionic gonadotropin, its .beta. subunit and .beta. core fragment in the urine: application to malignancies. Cancer Research 48:1361-1366 (Exhibit M).
Pettersson, K. et al. 1991. Monoclonal antibody-based discrepancies between two-site immunometric tests for lutropin. Clin. Chem. 37(10:Pt. 1): 1745-1748 (Exhibit N).
Prentice, L.G. and Ryan, R.J. 1975. LH and its subunits in human pituitary, serum and urine. J. Clin. Endocrinol. Metab. 40:2 303-312 (Exhibit O).
Romani, P. et al. 1977. Biologically active luteinizing hormone (LH) in plasma: II. Comparison with immunologically active LH levels throughout the human menstrual cycle. Acta Endocrinol (Copenh) 84:4 697-712 (Exhibit P).

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