DNA analyzer

Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus

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204621, G01N 2726

Patent

active

059763388

ABSTRACT:
Arranging a pair of temperature control units on both sides of gel 1 and arranging light-transmitting slit 134 on one of the elements, irradiation of an excitation beam over the gel 1 and signal detection are practiced through the slit 134. Introducing dry air onto the slit part, mildew occurrence is prevented on the detecting part. Detecting the power level applied to the gel and calculating the temperature of the temperature control units so that the gel temperature might be a predetermined temperature, on the basis of the detected power level, thereafter carrying out the feedback control of the power level, the gel temperature can be controlled appropriately and strictly during electrophoresis in an automatic fluorescent electrophoresis system, so that high-speed analysis can be done highly reproducibly at a higher voltage applied even by SSCP.

REFERENCES:
patent: 3719580 (1973-03-01), Roberts et al.
patent: 5360523 (1994-11-01), Middendorf et al.
"Alterations of the p53 Gene are Common and Critical Events for the Maintenance of Malignant Phenotypes in Small-Cell Lung Carcinoma", Oncogen(month unknown 1992) 7,451-457. Y. Sameshima, et al.
"Rapid and Sensitive Detection of Point Mutations and DNA Polymorphisms Using the Polymerase Chain Reaction", by M. Orita, et al, Genomics 5, 874-879 (month unknown 1989).
"Heat Transfer" edited by J. P. Holman. pp. 38-39. McGraw Hill 1989 month unknown.

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