Accurate mass determination with maldi time-of-flight mass spect

Details Accurate mass determination with maldi time-of-flight mass spect Accurate mass determination with maldi time-of-flight mass spect

1997-08-25

1999-03-23

Berman, Jack I.

Radiant energy

Ionic separation or analysis

Methods

250287, 2502521, H01J 4940

Patent

active

058863451

ABSTRACT:
The invention relates to the exact mass determination of analyte ions in time-of-flight mass spectrometers with ionization of analyte substances on sample supports by matrix-assisted laser desorption (MALDI), and with an improvement in mass resolution by delayed ion acceleration in the field between the sample support and an intermediate electrode. It particularly relates to methods for subsequent correction of the flight time values if, during the scan, an incorrect adjustment of the distance from the sample support to the nearest acceleration electrode has occurred.
The invention obtains a correction parameter for the flight times of all ions in the spectrum from an internal reference substance and corrects all flight times using a quadratic correction equation, before using the calibrated mass scale for the calculation of the exact masses. The quadratic correction equation contains a control parameter which multiplicatively affects both the linear and quadratic correction term. If no better suitable reference ions are available, oligomeric ions from the matrix of the MALDI method may be used as reference ions. The method can be applied regardless of adjustment in the optimum focus range before measurement of the spectrum, if the adjustments were made in a particular manner.

REFERENCES:
patent: 5504326 (1996-04-01), Reilly et al.
Robert S. Brown et al, Mass Resolution Improvement by Incorporation of Pulsed Ion Extraction in a Matrix-Assisted Laser Desorption/Ionization Linear Time-of-Flight Mass Spectrometer, Analytical Chemistry, vol. 67, No.13, pp. 1998-2003, Jul. 1, 1995.
Johann M. Grundwurmer et al., High-resolution mass spectrometry in a linear time-of-flight mass spectrometer, International Journal of Mass Spectrometry and Ion Processes, vol., 131, pp. 139-148, 1994.

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