Process for acetylating seaweed alginate with pseudomonas syring

Details Process for acetylating seaweed alginate with pseudomonas syring Process for acetylating seaweed alginate with pseudomonas syring

1992-09-11

1994-05-03

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

43525234, 435874, C12P 1904, C12N 120

Patent

active

053087615

ABSTRACT:
A method of biosynthetically acetylating seaweed alginates by modification with certain Pseudomonas syringae, such as P. syringae subsp. phaseolicola ATCC 19304, is disclosed. Acetylation occurs almost entirely in the 0-2 and 0.3 positions of the mannuronic acid residues. The acetylated alginates have several desirable properties. For example, acetylation increases the polymers' viscosity; it increases the flexibility of their gels; and it can produce a strong, thermoreversible-gel network. Acetylation increases the viscosity of the polymer, decreases ion-binding capacity, and decreases the ability to gel with calcium. The degree of acetylation can be controlled by controlling the exposure time, which allows the properties of the resulting polymer to be custom-made.

REFERENCES:
patent: 3856625 (1974-12-01), Imrie
patent: 3905869 (1975-09-01), Hidaka et al.
patent: 4235966 (1980-11-01), Jarman et al.
patent: 4490467 (1984-12-01), Jarman et al.
Gross et al., "Demonstration of Levan and Alginate in Bean Plants (Phaseolus vulgaris) Infected by Pseudomonas syringae pv. phaseolicola," J. Phytopathol., vol. 120, No. 1, pp. 9-19 (1987).
Gross et al., "Studies on the Extracellular Polysaccharides (EPS) Produced in vitro by Pseudomonas phaseolicola. I. Indications for a Polysaccharide Resembling Alginic Acid in Seven P. syringae Pathovars," J. Phytopathol. vol. 118, No. 3, pp. 276-287 (1987).
Gross et al., "Studies on the Extracellular Polysaccharides (EPS) Produced in vitro by Pseudomonas phaseolicola. II. Characterization of Levan, Alginate, and `LPS`," J. Phytopathol., vol. 119, No. 3, pp. 206-215 (1987).
Gross et al., "Studies on the Extracellular Polysaccharides (EPS) Produced in vitro by Pseudomonas phaseolicola. III. Kinetics of Levan and Alginate Formation in Batch Culture and Demonstration of Levansucrase Activity in Crude EPS," J. Phytopathol., vol. 119, No. 4, pp. 289-297 (1987).
Fett et al., Appl. Environ. Microbiol., 52(3), 466-73, 1986.
Osman et al., J. Bacteriol., 166(1), 66-71, 1986.
Fett et al., Plant Physiol., 69(1), 5-9, 1989.
Gross et al., Plant Pathog. Bact., Proc. Int. Conf., 6th Meeting Date 1985, 523-9, 1987.
Siugh et al., Microbios., 51(206), Jul. 13, 1987.
Skjak-Braek et al., "Monomer Sequence and Acetylation Pattern in Some Bacterial Alginates," Carbohydrate Research, vol. 154, pp. 239-250 (1986).
Pindar et al., "The Biosynthesis of Alginic Acid by Azotobacter vinelandii," Biochem. J., vol. 152, pp. 617-622 (1975).
Skjak-Braek et al., "Selective Acetylation of Mannuronic Acid Residues in Calcium Alginate Gels," Carbohydrate Research, vol. 185, pp. 119-129 (1989).

Affiliated with

Also associated with

Rating

Process for acetylating seaweed alginate with pseudomonas syring does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for acetylating seaweed alginate with pseudomonas syring, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for acetylating seaweed alginate with pseudomonas syring will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2114466