Rapid synthesis and analysis of carbohydrates

Details Rapid synthesis and analysis of carbohydrates Rapid synthesis and analysis of carbohydrates

1992-10-30

1994-05-03

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041828, 204299R, 435 14, 435 18, 435 72, C25B 300

Patent

active

053084608

ABSTRACT:
The subject invention provides novel methods and apparatus for synthesizing carbohydrates of a desired structure. The subject synthesis methods include using carbohydrage-modifying enzymes to perform individual synthesis reaction steps and electrophoresis to purify the reaction products for possible further use as substrates in additional synthesis reactions. The subject invention also provides novel methods and apparatus for detecting carbohydrates of interest. The methods and apparatus for synthesizing or detecting carbohydrates involve the use of carbohydrate-modifying enzymes immobilized in a reaction matrix gel and fluorophore labelled substrates that may be removed from the reaction matrix gel by electrophoresis and subsequently analyzed or transferred to another reaction gel matrix.
The subject invention also provides methods for detecting and quantitating specific carbohydrate-modifying enzymes that might be present in a sample for analysis.

REFERENCES:
patent: 5100778 (1992-03-01), Rademacher et al.
patent: 5205917 (1993-04-01), Klock, Jr.

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