Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-05-09
2000-09-26
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 771, 435 772, 435 79, 435960, 435962, G01N 33535, G01N 33532, G01N 33545
Patent
active
061241091
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The field of the present invention is that of analytical chemistry, a field of interest, more particularly, for identification and analysis of chemical species, preferably biological species, and in particular high molecular weight species, such as nucleic acids, or also biopolymers of a protein nature, e.g.: enzymes/substrates and antigens/antibodies.
More precisely, the present invention relates to: substances, preferably biological substances, by amplified chemiluminescence,
Substances or biological species, more particularly but not limitatively, envisaged by the invention are the nucleic acids RNA and DNA and any genetic structure containing them, as well as compounds which are capable of being involved in immunological antigen (Ag)/antibody (Ab) reactions, and furthermore products which couple to one another in the context of a mechanism of recognition and enzymatic reaction: enzyme/substrate, enzyme/inhibitor, receptor/ligand and lectin/sugar.
PRIOR ART
To detect, identify or analyse this type of molecule or structure, it is conventional to use their property of bioaffinity, that is to say their peculiar capacity for coupling specifically with their complements by mechanisms of genetic hybridization or immunological or enzymatic recognition.
Chemiluminescence, and more specifically also that which is so-called amplified, is one of the analytical techniques which proceeds by this principle and which is currently used in the laboratory.
Detection by amplified chemiluminescence can be found in the context of well-known analytical procedures of the "western blot" type or of the "ELISA" type as regards proteins, or also "southern & northern blot" type as regards RNA and DNA.
This technique of amplified chemiluminescence is an advantageous and promising alternative compared with conventional immunological analyses in which radioactive isotopes are used as the marker and indicator of the coupling under consideration. In fact, radioanalysis is starting to become out-of-date, since it has a large number of disadvantages, including, in particular:
Unfortunately, the development of these analytical techniques by amplified chemiluminescence has been slowed down because of the limited sensitivity of the systems which currently exist. This sensitivity depends in part on the light emitted in the course of and at the end of the luminescence reaction which indicates the couplings under consideration.
At this stage of the description, it appears useful to state the concepts by recording the principles of detection by amplified chemiluminescence. Firstly, luminescence can be defined as being the emission of light resulting from the restoration of part of the energy emanating from a substance in an excited state. In chemiluminescence, the excitation results from a chemical reaction. The latter is triggered following coupling of a ligand, directly or indirectly, with one or more substances, preferably biological substances, to be analysed. This ligand is one of the elements of an analytical system comprising, in addition, a chemiluminescent reagent, an enzyme or catalytic subunits of chemiluminescence, and a substrate which is specific to this enzyme or the catalytic subunit or subunits and is capable of being converted under the effect thereof into at least one initiator of the excitation of the luminescent reagent, this excitation being accompanied by the production of light.
One of the systems used the most in amplified chemiluminescence is that at the heart of which is found chemical reactions of reagents of the cyclic diacylhydrazide type, such as luminol or isoluminol. In such a system, the enzymes used are, for example, peroxidases, which are capable of converting a substance called an oxidant, such as H.sub.2 O.sub.2, into an initiator of the oxidation of luminol. Since this oxidation produces too few photons, the excitation of luminol cannot be detected with a significant sensitivity, and it is therefore necessary to amplify this oxidation reaction.
Among the most effective amplifiers known,
REFERENCES:
patent: 4598044 (1986-07-01), Kricka et al.
patent: 5043266 (1991-08-01), Dewar et al.
patent: 5206149 (1993-04-01), Oyama et al.
Blum et al, NATO ASI Ser., Ser. 2 38 (Biosensors for Direct Monitoring of Environmental Pollutants in Field), 271-280 (1997).
Analytical Letter, vol. 27, No. 6, Jan. 1, 1994 pp. 1189-1122, XP 000568630, Hori et al, "A New . . . Luminometer".
Blum Loic
El Alaoui Said
Henry Robert
Ceperley Mary E.
Innogenetics N.V.
LandOfFree
System for qualitatively and/or quantitatively analyzing prefera does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System for qualitatively and/or quantitatively analyzing prefera, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for qualitatively and/or quantitatively analyzing prefera will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2098963