Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1990-03-06
1994-04-12
Housel, James C.
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
356307, 356317, 2504581, 436536, 436546, 436172, G01N 2164
Patent
active
053023498
ABSTRACT:
Light, pulsed or continuous at a particular wavelength (e.g. 780 nm), fluoresces a specimen. The specimen may be combinations of an antigen (e.g. rubella) labelled with a fluorescent dye, unlabeled antigen or hapten and an antibody reactive with the antigen or hapten. The light polarized in a first direction (e.g. z-axis) parallel to the electric field of the incident light and in a second direction (e.g. x-axis) perpendicular to the first direction is measured. A second specimen is then provided with the antigen and the antibody but without the dye. The same light as discussed above excites the second specimen and polarizes the light. The light polarized in the first (z-axis) and second (x-axis) directions in the second specimen is measured. These measurements are processed in a microprocessor with the measurements in the z and x directions in the first specimen to identify the antigen or, when the antigen is known, to identify the concentration of the antigen in the first specimen. When the light is pulsed, the measurements are made in a time window beginning after the initiation, and terminating before the end, of the fluorescence of the combination of the dye, the antibody and the antigen. Determinations as discussed above but without unlabeled antigen or hapten may be made of the antibody instead of the antigen. When the light is continuous, it is modulated. Measurements are made of the phase shifts in the polarized light in the z and x directions as a result of the light modulations and the decay of the fluorescence.
REFERENCES:
patent: 4131800 (1978-12-01), Bruck et al.
patent: 4341957 (1982-07-01), Wieder
patent: 4419583 (1983-12-01), Noeller
patent: 4555177 (1985-12-01), Barrett
patent: 4786170 (1988-10-01), Groebler
patent: 4877965 (1989-11-01), Dandliker et al.
Dissertation Abstracts International Abstract vol. 50/10-B p. 4497 (1989).
Dissertation Abstracts International Abstract vol. 44/03-B p. 814 (1983).
Dandliker June K.
Dandliker Walter B.
Levin Jacques C.
Diatron Corporation
Housel James C.
Roston Ellsworth R.
Schwartz Charles H.
Snay Jeffrey R.
LandOfFree
Transient-state luminescence assay apparatus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Transient-state luminescence assay apparatus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transient-state luminescence assay apparatus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2096842