Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1985-09-03
1988-08-30
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 29, 435 34, 436531, 436532, 536 27, G01N 33545, G01N 33554
Patent
active
047677015
ABSTRACT:
A carrier which consists of a carboxyl group-containing polymer, preferably activated polymethyl methacrylate, and on the surface of which a biologically active substance consisting of Poly I:C is immobilized, is described, as is a method of preparing such a carrier, and its use for diagnostic purposes. In the method of immobilizing Poly I:C on a carrier surface of polymethyl methacrylate, the surface of the polymethyl methacrylate carrier is first activated by treating it with strong alkali, such as a 10M sodium hydroxide solution. An aqueous solution of Poly I:C is heated, preferably for 1-2 h, and is then allowed to cool to about 50.degree.-80.degree. C., whereupon a coupler, preferably a water-soluble carbodiimide, is added to the solution. The warm solution is then applied to the activated carrier surface to immobilize Poly I:C on the carrier surface which then is allowed to cool to 0.degree.-50.degree. C.
REFERENCES:
patent: 4352884 (1982-10-01), Nakashima
patent: 4357142 (1982-11-01), Schall
patent: 4362697 (1982-12-01), Tabb
patent: 4418152 (1983-11-01), Hosaka
patent: 4559303 (1985-12-01), Aotani
Chemical Abstracts, I, 101:192407m (1984).
Chemical Abstracts, II, 101:235572f (1984).
Holmberg Krister
Hyden Holger
Berol Kemi AB
Marantz Sidney
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