Analytical enzymatic determination

Details Analytical enzymatic determination Analytical enzymatic determination

1975-03-10

1977-04-26

Shapiro, Lionel M.

Textiles: fiber preparation

G01N 3114

Patent

active

040199614

ABSTRACT:
An analytical process for enzymatic determination of a substrate in biological fluids. The fluid sample is reacted first in the absence of the specific kinase but in the presence of an NADH -- regenerating enzymatic system with the reagents needed for the reaction sequence; then the enzymes are separated from the reaction solution, and thereafter the necessary enzymes are added to the filtrate and NADH consumption is measured.
The method is very accurate, economical in requiring less enzymes and only one measurement is necessary.

REFERENCES:
patent: 3526480 (1970-09-01), Findl et al.
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3862009 (1975-01-01), Wahlefeld et al.
R. Bonnichsen, "Ethanol" H. U. Bergmeyer, Methods of Enzymatic Analysis, Academic Press, N.Y. and London, pp. 285-287, (1965).

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