G-banding of plant chromosomes

Details G-banding of plant chromosomes G-banding of plant chromosomes

1983-11-21

1986-06-24

Bagwill, Robert E.

Plant husbandry

Process

424 3, 435 6, 435317, A01G 100

Patent

active

045960895

ABSTRACT:
A method for the staining of the chromosomal material of plant cells so as to induce G-Bands, useful in plant genetic studies such as hydridization. G-Banding is induced by contacting living plant cells, such as those of plants of the family Gramineae, with actinomycin D, N-methyl-N'-nitroguanidine or ethidium bromide. More particularly, plant cells are treated with AMD and colchicine before fixation, prepared for viewing through a microscope by staining and then mounted on a glass slide.

REFERENCES:
93 Chemical Abstracts, 40643p (1980).
Sumner, A. T., "Dye Binding Mechanisms in G-Banding of Chromosomes", Journal of Microscopy, vol. 119, pt. 3 (1980), pp. 397-406.
Taniguchi, K. et al., "Banding Techniques for Plant Chromosomes", Scibo, vol. 9, No. 4 (1977), pp. 126-131.
Schweizer, D., "DAPI Fluorescence of Plant Chromosomes Prestained with Antinomycin D", Exp. Cell. Res., vol. 102 (1976), pp. 408-413.
Hsu, T. C. et al., "Induction of Chromosome Crossbanding by Treating Cells with Chemical Agents Before Fixation", ExpH. Cell Res., vol. 79 (1973), pp. 484-487.

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