Radioimmune method of assaying quantitatively for a hapten

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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23230B, A61k 2704

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039404757

ABSTRACT:
A radioimmune method of assaying quantitatively for a hapten comprising contacting an aqueous sample containing the hapten under assay, a known quantity of a radioactively labeled marker which incorporates a hapten homologous to the hapten under assay, and an insolubilized antibody specific for the hapten under assay to competitively bind part of said labeled hapten and the hapten contained in said sample to the antibody; incubating the foregoing mixture until a two-phase system is produced comprising an insoluble or solid phase and an aqueous phase, and measuring the radioactivity of at least one of said two phases, the value of said radioactivity being a function of the concentration in said sample of the hapten under assay.

REFERENCES:
patent: 3555143 (1971-01-01), Axen et al.
patent: 3646346 (1972-02-01), Catt
Goodfriend et al., J. Lab. & Clin. Med. Vol. 72, Oct. 1968, pp. 648 to 662.
Walsh et al., The Journal of Infectious Diseases, Vol. 121, No. 5, May 1970, pp. 550-554.
Wide, Radioimmuno Assay Methods, Editors Kirkham and Hunter, (1970) pp. 405-412.

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