Microbiological synthesis of beta endorphin

Details Microbiological synthesis of beta endorphin Microbiological synthesis of beta endorphin

1980-03-10

1982-09-21

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 68, 435 70, 435172, 435253, 435317, 435820, 435848, 435849, 2601125R, C12P 2102, C12N 1500, C12N 120, C12N 100

Patent

active

043507646

ABSTRACT:
DNA comprising the naturally occurring nucleotide sequence coding for amino acids 44-90 of .beta.-lipotropin and including the entire coding region for .beta.-endorphin with the exception of the C-terminal glutamine was modified, transferred to an expression transfer vector, and expressed as a fusion protein. The fusion protein was further modified in vitro to yield mature .beta.-endorphin. .beta.-endorphin was purified from a bacterial lysate. The structure and biological activity of the resulting product was proven by immunological assay, and by two independent assays designed to demonstrate biological activity.

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