Method for the separation of a protein substance from a solution

Details Method for the separation of a protein substance from a solution Method for the separation of a protein substance from a solution

1980-06-17

1982-09-21

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 210635, 424 12, 435 26, G01N 3354

Patent

active

043507603

ABSTRACT:
The invention relates to a method for the selective separation of a given protein substance having an affinity for a ligand from other substances some of them having the same affinity for the ligand but also having a high molecular weight, higher than that of said protein substance, said substances being in solution.
The method consists of filtering said substances on a gel which excludes said high molecular weight substances, said gel being coupled with a ligand for which said protein substance has affinity.
The method may be applied to enzymatic and especially to immunoenzymatic assays.

REFERENCES:
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patent: 3879262 (1975-04-01), Schuurs et al.
patent: 3917527 (1975-11-01), Shaltiel
patent: 3948728 (1976-04-01), Roeschlau et al.
patent: 4125492 (1978-11-01), Cuatrecasas et al.
patent: 4138474 (1979-02-01), Updike
patent: 4205130 (1980-05-01), Vihko
patent: 4235960 (1980-11-01), Sasse et al.
patent: 4273865 (1981-06-01), von Stetten et al.
Baum et al. "Affinity Chromatography" Chapter 8, Immobilized Enzymes Antigens, Antibodies, & Peptides, Marcel Dekker Inc., N.Y., pp. 419-425.

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