Stable protein-nickel particles and methods of production and us

Details Stable protein-nickel particles and methods of production and us Stable protein-nickel particles and methods of production and us

1997-10-09

2000-06-13

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

427 211, 427 213, 427 214, 427131, 42721335, 427215, 427216, 427337, 427338, 428403, 428407, 435 5, 435 721, 436524, 436525, 436526, 436529, 436531, 436532, 436533, 436534, G01N 33533

Patent

active

060748842

ABSTRACT:
A stable protein-coated nickel particle useful in biological assays contains a nickel particle having removed from the surface thereof nickel oxide; a linker attached to said nickel particle, the linker having a free amino group; and a protein attached to said linker by covalently bonding to the free amino group. Methods of producing and using these oxide-free nickel-protein conjugates are disclosed.

REFERENCES:
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G. Hermanson, "Bioconjugate Techniques", Academic Press (1996).
M. Aslam et al, "Bioconjugation--Protein Coupling Techniques for the Biomedical Sciences", Grove's Dictionaries, Inc. (1998).
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C. Kittel et al, "Ferromagnetic Domain Theory", in Solid State Physics, F. Seitz and D. Turnbull, eds., vol. 3, pp. 437-564, New York, NY (1956).
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J. Whitesell et al, "Directionally Aligned Helical Peptides on Surfaces", Science, 261:73-76 (Jul. 2, 1993).

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