HBV polymerase processes for preparation and uses for screening

Details HBV polymerase processes for preparation and uses for screening HBV polymerase processes for preparation and uses for screening

1997-08-15

1999-10-19

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435194, 4353201, 43525233, 536 234, 536 232, C12N 912, C12N 1554, C12N 1562

Patent

active

059687819

ABSTRACT:
The present invention relates to hepatitis B virus (hereinafter it refers to HBV) polymerase containing a histidine tag, RNase H enzyme derived from HBV polymerase and processes for preparation thereof.
More particularly, the present invention relates to recombinant HBV polymerase, its RNase H domain with enzyme activity, expression vectors producing the enzymes in E. coli and processes for preparing the HBV polymerase and the RNase H enzyme which can be easily purified due to their histidine tags.
And the present invention relates to uses of the HBV polymerase and the RNase H enzyme for screening antiviral agents.

REFERENCES:
Muller et al., "Co-expression of the Subunits of the Heterodimer of HIV-1 Reverse Transcriptase in Escherichia coli", J. Bio. Chem 264, No. 24, pp. 13975-13976 (Aug. 1989).
Tan et al., "Inhibition of the RNase H Activity of HIV Reverse Transcriptase by Azidothymidylate", Biochemistry 30, No. 20, pp. 4831-4835 (May 1991).
Smith, et al., "Purification and Characterization of an Active Human Immunodeficiency Virus Type 1 RNase H Domain", Journal of Virology 67, No. 7, pp. 4037-4049 (Jul. 1993).

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