Purified telomerase

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Transferases

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435194, 4352523, 4353201, 435 913, 536 232, 935 8, 935 9, 935 14, 530412, 530413, A61K 3851, C12N 912, C07H 2104, A23J 100

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059685069

ABSTRACT:
This invention provides purified human telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of a first matrix that binds molecules bearing negative charges, a matrix that binds molecules bearing positive charges, a second matrix that binds molecules bearing negative charges, an affinity purification step and a matrix that separates molecules according to their size.

REFERENCES:
patent: 5583016 (1996-12-01), Villeponteau
Counter et al. PNAS, USA. 91: 2900-2904, Apr. 1994.
Feng, Junli et al., "The RNA Component of Human Telomerase," Science, vol. 269, No. 5228, Sep. 1, 1995, pp. 1236-1241.
Nomura, Nobou, et al., "Prediction of the Coding Sequences of Unidentified Human Genes," DNA Research, vol. 1, 1994, Japan, pp. 27-35.
Srivastava, Meera et al., "Genomic Organization and Chromosomal Localization of the Human Nucleolin Gene," Journal of Biological Chemistry, vol. 265, No. 25, 1990, pp. 14922-14931.
Bochnig, P. et al., "A Monoclonal Antibody Against 2,2,7-trimethylguanosine that reacts with intact class U, small, nuclear ribonucleoproteins as well as with 7-methyl-guanosine-caped RNAs," Eur.J.Biochem. 168, pp. 461-467, 1987.
Database WPI, XP002049270 and JP 09 145 575 A (Sosei KK), Section Ch, Week 9734, Derwent Publications, Ltd., GB; Class B04, AN 97-367058, Jun. 17, 1997.
Database WPI XP002049271 and JP 07 242 566 A (Kirin Brewery KK), Section Ch, Week 9546, Derwent Publications, Ltd., London, GB; Class B04, AN 95-355193, Sep. 19, 1995.
M. Singer and D. Gottschling (1994) Science 266:404-409. "TLC1: Templates RNA Component of Saccharomyces cerevisiae Telomerase".
G. B. Morin (1989) Cell 59:521-529. "The Human Telomere Terminal Transferase is a Ribonucleoprotein That Synthesizes TTAGGG Repeats".
Graham et al. (1977) J. Gen. Virol. 36: 59-77. "Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5".
Stillman and Guzman (1985) Mol. and Cell Bio. 5:2051-2060. "Replication and Supercoiling of Simian Virus 40 DNA in Cell Extracts from Human Cells".
E.H. Blackburn (1991) TIBS 16:378-381 "Telemeres".
Miller and Rosman (1992) Biotechniques 7:980-990. "Improved Retroviral Vectors for Gene Transfer and Expression".
E.H. Blackburn (1992) Annu. Rev. Biochem. 61:113-29 "Telomerases".
C.B. Harley (1991) Mutation Research 256:271-282. "Telomere loss: mitotic clock or genetic time bomb?".
Romero and Blackburn (1991) Cell 67:343-353. "A Conserved Secondary Structure for Telomerase RNA".
J. Lingner et al. (1994) Genes and Devel. 8:1984-1988. "Telomerase RNAs of different ciliates have a common secondary and a permuted template".

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