Method for producing L-3,4-dihydroxyphenylalanine by precipitati

Details Method for producing L-3,4-dihydroxyphenylalanine by precipitati Method for producing L-3,4-dihydroxyphenylalanine by precipitati

1994-07-28

1996-05-21

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435847, C12P 1322

Patent

active

055189059

ABSTRACT:
A method for producing L-3,4-dihydroxyphenylalanine comprising contacting microorganism cells having .beta.-tyrosinase activity with catechol, pyruvic acid and ammonium ion or with catechol and L-serine, at a temperature lower than 25.degree. C. in the presence of anhydrous crystals of L-3,4-dihydroxyphenylalanine. L-3,4-dihydroxyphenylalanine formed from said contacting precipitates as anhydrous crystals in the reaction mixture, and the thus-precipitated anhydrous crystals can be collected. According to the method of the present invention, crude crystals of L-3,4-dihydroxyphenylalanine having a high purity may be obtained at a high yield of recovery.

REFERENCES:
patent: 3813317 (1974-05-01), Benoiton et al.
patent: 5338672 (1994-08-01), Tsuchida
Perry's Chemical Engineer's Handbook, pp. 19-24 to 19-40, Publishers: McGraw Hill, Inc., 1984.
Kirk-Othmer Encyclopedia of Chemical Technology, vol. 7, Publishers: John Wiley & Sons, Inc. pp. 243-285, 1979.
Patent Abstracts of Japan, vol. 17, No. 482 (C-1105)(6111), Sep. 2, 1993, JP-A-5 123177, May 21, 1993.

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