Transfected cell lines expressing autoantigens and their use in

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 71, 435 721, 435 693, 435 703, 4352402, 435975, 536 235, C12Q 168, G01N 3353, G01N 33567

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055188818

ABSTRACT:
This invention relates to improved in vitro immunoassay methods for detection of autoantibodies associated with autoimmune disease. More specifically, the invention relates to immunoassay methods which utilize a cell line stably transformed with a nucleic acid expressing an autoimmune antigen. This invention also relates to compositions comprising these cell lines and to kits containing such cell lines.

REFERENCES:
patent: 4751181 (1988-06-01), Keene
patent: 4784942 (1988-11-01), Harley
Veldhoven, C. H. A., et al. (1992) "The development of a quantitative assay for the detection of anit-Ro/SS-A and anti-LA/SS-B autoantibodies using purified recombinant proteins", Journal of Immunological Methods, 151:177-189.
Huff, John P., et al. (1990) "Insights Into Native Epitopes of Proliferating Cell Nuclear Antigen Using Recombinant DNA Protein Products", J. Exp. Med. 172:419-429.
Dickey, Darryl W., et al. (1993) "Presence of Anti-La(SS-B) Is Associated With Binding To The 13-kD Carboxyl Terminus of 60-kD Ro(SS-A) in Systemic Lupus Erythematosus", Journal of Investigative Dermatology, 100(4):412-416.
McCauliffe, D. P., et al. (1993) "Molecular characterization of the Ro/SS-A autoantigens", Journal of Investigative Dermatology, 100(1):73-79.
Buyon, Jill P., et al. (1994) "Autoantibody Responses to the Native 52-KDa SS-A/Ro protein in Neonatal Lupus syndromes, systemic Lupus Erythematosus and Sjogren's Syndrome", Journal of Immunology, 3675-3684.
Itoh, K., et al., (1991) "Protein heterogeneity in the human Ro/SSA ribonucleoproteins: The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes," J. Clin. Invest., 87:177-186.
Harley, J. B., et al., (1992) "Anti-Ro in Sjogren's syndrome and systemic lupus erythematosus," Rheumatic Disease Clinics of N. America, 18:337-358.
Gottlieb, E., et al., (1989) "The RNA binding protein La influences both the accuracy and efficiency of RNA polymerase III transcription in vitro," EMBO J., 8:841-850.
Gottlieb, E., et al., (1989) "Function of mammalian La protein evidence for its action in transcription termination by RNA polymerase III," EMBO J., 8:851-861.
Deutscher, et al., (1988) "Molecular analysis of the 60 kda human Ro ribonucleoprotein," Proc. Natl. Acad. Sci. USA, 85:9479-9483.
McNeilage, L. J., et al., (1990) "Mapping of epitopes on the La(SS-B) autoantigen of primary Sjogren's syndrome: identification of a cross-reactive epitope," J. Immunol., 145:3829-3835.
Germain, R. N., et al., (1986) "Analysis of the expression of Class II major histocompatibility complex-encoded molecules by DNA-mediated gene transfer," Annual Reviews in Immunology, 4:281-315.
Harmon et al., Arthritis and Rheumatism, vol. 27, No. 2, "The Importance of Tissue Substrate in the SS-A/Ro Antigen-Antibody System". Feb. 1984, pp. 166-173.
Chan et al, Journal of Clinical Investigation, vol. 87, "Molecular Definition and Sequence Motifs of the 52-kD Component of Human SS-A/Ro Autoantigen", Jan. 1991, pp. 68-76.
Rother et al, Clin. Exp. Immunol., vol. 83, "La/SSB Ribonucleo-Protein Levels Increased in Transformed Cells", 1991, pp. 369-374.
McCauliffe et al. J. Clin. invest., vol. 85, "Molecular Cloning, Expression, and Chromosome 19 Localization of Human Ro/SS-A Autoantigen", May 1990, pp. 1379-1391.
Barakat et al, Clin. Exp. Immunol, vol. 89, "IgG antibodies From Patients With Primary Sjogren's Syndrome and Systemic lupus Erythematosus Recognize Different Epitopes in 60-kD SSA/Ro protein", 1992, pp. 38-45.
Slobbe et al, Clin. Exp. Immunol., vol. 86, "Detection and Occurrence of the 60- and 52-kD Ro(SS-A) antigens and of Autoantibodies Against These Proteins", 1991, pp. 99-105.
Thomsen et al., Proc. Natl. Acad. Sci, USA, vol. 81, "Promoter-Regulatory Region of the Major Immediate Early Gene of Human Cytomegalovirus", Feb. 1984, pp. 659-663.

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