Emission quenching sensors

Details Emission quenching sensors Emission quenching sensors

1995-05-30

1996-05-21

Snay, Jeffrey R.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

422 91, 2504581, G01N 2164

Patent

active

055186947

ABSTRACT:
The present invention relates to a sensing instrument and method for measuring the concentration of an analyte. The sensing instrument includes: a sensing element including at least a first emissive indicator characterized by a bimolecular quenching rate constant k.sub.q, one or more fluorescence lifetimes .tau..sub.o above a lowest lifetime .tau..sub.oL, and capable of emitting analyte concentration dependent signals when exposed to an excitation signal in the presence of quencher; an excitation system which provides an amplitude modulated excitation signal at one or more radial modulation frequencies .omega.; a detector; and a processor. The processor univariantly processes the detected signals and provides output signals representative of analyte concentration. The sensing element and/or excitation system are configured to enable the instrument to operate sufficiently within the condition [(k.sub.q [Q]).sup.2 +.omega..sup.2 ].tau..sub.o.sup.2 >>1+2k.sub.q .tau..sub.o [Q] such that a slope of the relationship between the concentration dependent parameter and analyte concentration is independent of .tau..sub.o variability and/or heterogeneity for all analyte concentrations within the operating range and for all lifetimes .tau. .sub.o greater than .tau..sub.oL.

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