Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-05-26
1991-04-09
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 29, 435 91, 435803, 436501, 436519, 436543, 436177, 436178, 436815, 436825, 536 27, 935 19, 935 77, C12Q 168, G01N 33533
Patent
active
050064606
ABSTRACT:
A method of measuring chain breakage in the DNA of a eucaryotic cell is disclosed. This method includes (a) contacting the cell with a stripping solution that lyses and solubilizes the cell without denaturing its DNA, thereby forming a nucleoid having a halo, (b) measuring the width of the halo, and (c) determining the number of chain breaks from the measured width. The halo includes at least one loop of undenatured DNA, and has a width related to the number of DNA chain breaks in the loop. The cell to be examined may be adhered to a support prior to its contact with the stripping solution. The number of DNA chain breaks can be determined by compairing the measured width of the nucleoid halo with a reference value.
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Thomas Eric A.
Thomas, Jr. Charles A.
Marschel Ardin H.
Pantox Corporation
Wax Robert A.
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