Recombinant DNA expression vectors and DNA compounds which encod

Details Recombinant DNA expression vectors and DNA compounds which encod Recombinant DNA expression vectors and DNA compounds which encod

1986-08-08

1989-12-05

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

4351723, 4352522, 4352523, 43525233, 43525235, 435254, 435320, 536 27, 935 14, 935 27, 935 34, 935 60, 935 68, 935 72, C12N 900, C12N 1500, C12P 102, C12P 104

Patent

active

048852511

ABSTRACT:
The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.

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