Process for producing monoclonal antibodies which specifically b

Details Process for producing monoclonal antibodies which specifically b Process for producing monoclonal antibodies which specifically b

1989-12-28

1993-09-14

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351722, 435 7021, 53038885, 5303888, 5303877, C12N 520, C12N 1502, C12P 2108, C07K 1528

Patent

active

052448010

ABSTRACT:
Monoclonal antibodies manifesting specific reactivity to benign adenomatous polyps of the human colon are produced. Hybridoma technology was utilized to produce immunoglobulin producing cell lines. Splenic lymphocytes from mice, immunized with a membrane fraction derived from human tubular adenomatous colonic polyps, were fused with a mouse non immunoglobulin secreting myeloma cell line (NS-1). Screening of hybridoma culture supernatant for positive reactivity with adenomatous colonic polyp extract, and negative activity with extracts from normal liver and spleen allowed for selection of desired immunoglobulin producing cultures. Further screening of expanded cultures with extracts of colorectal cancer, apparently normal mucosa adjacent to the cancer, and a variety of normal human extracts allowed further distinctions in binding reactivity. One such selected cell line produced a monoclonal antibody designated Adnab-9 selected on this basis. Adnab-9 was reacted with colonic-washing effluent derived from patients undergoing routine precolonoscopic bowel cleansing and the resultant mean binding activity in the effluent of patients proved to have a colorectal cancer was significantly elevated with respect to those with an apparently normal colon. Since Adnab-9 binding may be increased in some colorectal cancer patients with early stage disease, Adnab-9 aids in the diagnosis, and timely treatment of colorectal cancer.

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