Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Inactivation or attenuation; producing viral subunits
Patent
1980-11-19
1982-07-27
Rosen, Sam
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Inactivation or attenuation; producing viral subunits
424 89, C12N 708, A61K 3912
Patent
active
043418708
ABSTRACT:
This invention relates to a precursor or intermediate for a rotavirus vaccine to protect infants and young children against diarrhea caused by rotavirus. The invention is the efficient propagation of a strain of human rotavirus type 2 prepared by a method of cultivation by multiple passages in vivo in gnotobiotic piglets and by multiple passages in vitro subsequently in AGMK (African green monkey kidney) cell cultures. Additionally, rotavirus is treated with a biologically active amount of trypsin prior to growth in AGMK cells, and the inoculated cell cultures are centrifuged at a low centrifugal force before incubation.
REFERENCES:
McNulty-J. Gen. Virol., vol. 40, No. 1, (1978) pp. 1 & 6-8.
Wyatt et al., Perspectives in Virology, vol. 10, Raven Press, New York, 1978, pp. 121-145.
Kapikian et al., The Am. Journal of Clinical Nutrition, vol. 31, Dec. 1978, pp. 2219-2236.
Babiuk et al., Journal of Clinical Microbiology, 6(6):610-617, Dec. 1977.
Theil et al., American Journal Veterinary Research, 38:1765-1768, 1977.
Almeida et al., Journal of General Virology, 40:213-218, 1978.
Kapikian, et al., Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 5th ed., Lennette et al. (eds.), Am. Public Health Assoc., Wash., DC, pp. 941-942, 1979.
Barnett et al., Journal of Clinical Microbiology, 10:111-113, 1979.
McNulty et al., Archives of Virology, 61:13-21, 1979.
Drozdov et al., Vopr. Virusol., 4:389-92, 1979.
Theil et al., American Journal of Veterinary Research, 41:140-143, 1980.
Wyatt et al., Science, 207:189-191, Jan. 11, 1980.
Kapikian et al., Reviews of Infectious Diseases, 2(3):459-469, 1980.
Bohl Edward H.
Chanock Robert M.
Greenberg Harry B.
James Walter D.
Kalica Anthony R.
Roberts, Jr. John S.
Rosen Sam
The United States of America as represented by the Secretary of
LandOfFree
Cultivatable human rotavirus type 2 does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cultivatable human rotavirus type 2, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cultivatable human rotavirus type 2 will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2025935